cell cycle regulators Search Results


cells  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank cells
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anti cdkn2a p16ink4a affinity  (MedChemExpress)
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MedChemExpress anti cdkn2a p16ink4a affinity
Anti Cdkn2a P16ink4a Affinity, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk2  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc cdk2
(A–D) Stable A2780, SKOV3 cells with TSPAN12 silencing were analyzed by western blot analysis of Cyclin A2, Cyclin E2, Cyclin D1, <t>CDK2,</t> and CDK4. (E-H) OVCAR3 and SKOV3 cells with TSPAN12 overexpression were analyzed by western blot analysis after being treated with or without CDK inhibitor, AT7519 (Inh, 100 nM) for 72 h. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are presented as the mean ± standard deviation (SD) of three independent experiments.
Cdk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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g1 s cell cycle checkpoint  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc g1 s cell cycle checkpoint
(A–D) Stable A2780, SKOV3 cells with TSPAN12 silencing were analyzed by western blot analysis of Cyclin A2, Cyclin E2, Cyclin D1, <t>CDK2,</t> and CDK4. (E-H) OVCAR3 and SKOV3 cells with TSPAN12 overexpression were analyzed by western blot analysis after being treated with or without CDK inhibitor, AT7519 (Inh, 100 nM) for 72 h. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are presented as the mean ± standard deviation (SD) of three independent experiments.
G1 S Cell Cycle Checkpoint, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdkn2a  (ProSci Incorporated)
90
ProSci Incorporated cdkn2a
Genetic alterations in NPC-PDX tumors. ( a ) Copy number variations (CNV) of NPC-PDX tumors versus corresponding patient’s peripheral blood mononuclear cells (PBMC). Genome-wide CNV alterations in four paired PDX tumor samples (ST, LN, LG and LV). CCND1 CNV gain (red arrow) and <t>CDKN2A</t> CNV loss (green arrow) are indicated. ( b ) HE and EB virus-encoded small RNA (EBER) staining of parental NPC tumor with bone metastasis and its derived NPC-PDX. ( c ) CNV profile comparisons of NPC FFPE-Bone and PDX-Bone based on WES and ( d ) ultra-deep sequencing of cancer panel-409 are shown (genes associated with or without copy number alteration are indicated in different colors or in grey, respectively). Observed copy number for each evaluated position is shown on the y-axis as a log 2 scale. Correlation plots with Pearson’s correlation coefficient, r, indicating similarities between two CNV profiles
Cdkn2a, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho plk1 thr210  (ProSci Incorporated)
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ProSci Incorporated anti phospho plk1 thr210
Genetic alterations in NPC-PDX tumors. ( a ) Copy number variations (CNV) of NPC-PDX tumors versus corresponding patient’s peripheral blood mononuclear cells (PBMC). Genome-wide CNV alterations in four paired PDX tumor samples (ST, LN, LG and LV). CCND1 CNV gain (red arrow) and <t>CDKN2A</t> CNV loss (green arrow) are indicated. ( b ) HE and EB virus-encoded small RNA (EBER) staining of parental NPC tumor with bone metastasis and its derived NPC-PDX. ( c ) CNV profile comparisons of NPC FFPE-Bone and PDX-Bone based on WES and ( d ) ultra-deep sequencing of cancer panel-409 are shown (genes associated with or without copy number alteration are indicated in different colors or in grey, respectively). Observed copy number for each evaluated position is shown on the y-axis as a log 2 scale. Correlation plots with Pearson’s correlation coefficient, r, indicating similarities between two CNV profiles
Anti Phospho Plk1 Thr210, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amino acids 117 119  (ProSci Incorporated)
92
ProSci Incorporated amino acids 117 119
Comparison of non-structural protein 1 sequences among closely related flaviviruses. ( A ): Ribbon model highlighting regions of the NS1 protein containing segments exposed at the outer surface to the host environment. ( B ): Sequence comparison showing regions with high sequence disparity. Amino acids depicted in red differ from the corresponding ZIKV NS1 amino acids. A represents positions with two sequences with amino acids identical to ZIKV NS1. The boxes highlight highly conserved sequences, amino acids 117–119 and 227–229, that were mutated to alanine in immunodominant regions 2 and 3.
Amino Acids 117 119, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclins and cell cycle regulation  (BioCarta)
90
BioCarta cyclins and cell cycle regulation
Top ten pathways identified by pathway enrichment analysis for miR-30a and -191 .
Cyclins And Cell Cycle Regulation, supplied by BioCarta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdk cell cycle regulator  (Medicago)
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Medicago cdk cell cycle regulator
Top ten pathways identified by pathway enrichment analysis for miR-30a and -191 .
Cdk Cell Cycle Regulator, supplied by Medicago, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qpcr screening kit for cell cycle-regulating genes  (Bioneer Corporation)
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Bioneer Corporation qpcr screening kit for cell cycle-regulating genes
Top ten pathways identified by pathway enrichment analysis for miR-30a and -191 .
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p19 cell cycle regulators/markers  (Novocastra)
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Novocastra p19 cell cycle regulators/markers
Top ten pathways identified by pathway enrichment analysis for miR-30a and -191 .
P19 Cell Cycle Regulators/Markers, supplied by Novocastra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacterial cell cycle and growth phase switch by the essential transcriptional regulator ctra  (Viollier AG)
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Viollier AG bacterial cell cycle and growth phase switch by the essential transcriptional regulator ctra
Top ten pathways identified by pathway enrichment analysis for miR-30a and -191 .
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Image Search Results


(A–D) Stable A2780, SKOV3 cells with TSPAN12 silencing were analyzed by western blot analysis of Cyclin A2, Cyclin E2, Cyclin D1, CDK2, and CDK4. (E-H) OVCAR3 and SKOV3 cells with TSPAN12 overexpression were analyzed by western blot analysis after being treated with or without CDK inhibitor, AT7519 (Inh, 100 nM) for 72 h. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are presented as the mean ± standard deviation (SD) of three independent experiments.

Journal: Molecules and Cells

Article Title: TSPAN12 Precedes Tumor Proliferation by Cell Cycle Control in Ovarian Cancer

doi: 10.14348/molcells.2019.0015

Figure Lengend Snippet: (A–D) Stable A2780, SKOV3 cells with TSPAN12 silencing were analyzed by western blot analysis of Cyclin A2, Cyclin E2, Cyclin D1, CDK2, and CDK4. (E-H) OVCAR3 and SKOV3 cells with TSPAN12 overexpression were analyzed by western blot analysis after being treated with or without CDK inhibitor, AT7519 (Inh, 100 nM) for 72 h. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are presented as the mean ± standard deviation (SD) of three independent experiments.

Article Snippet: Blots were incubated with antibodies against TSPAN12 (1:400, anti-rabbit, Av46887; Sigma), Cyclin D1, Cyclin E2, CDK2, CDK4 (1:400, anti-rabbit, Cell Cycle Antibody Sampler kit #9932, #9870; Cell Signaling Technology), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:1,000, anti-mouse, 60004-1; Proteintech, USA).

Techniques: Western Blot, Over Expression, Standard Deviation

Genetic alterations in NPC-PDX tumors. ( a ) Copy number variations (CNV) of NPC-PDX tumors versus corresponding patient’s peripheral blood mononuclear cells (PBMC). Genome-wide CNV alterations in four paired PDX tumor samples (ST, LN, LG and LV). CCND1 CNV gain (red arrow) and CDKN2A CNV loss (green arrow) are indicated. ( b ) HE and EB virus-encoded small RNA (EBER) staining of parental NPC tumor with bone metastasis and its derived NPC-PDX. ( c ) CNV profile comparisons of NPC FFPE-Bone and PDX-Bone based on WES and ( d ) ultra-deep sequencing of cancer panel-409 are shown (genes associated with or without copy number alteration are indicated in different colors or in grey, respectively). Observed copy number for each evaluated position is shown on the y-axis as a log 2 scale. Correlation plots with Pearson’s correlation coefficient, r, indicating similarities between two CNV profiles

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Integrated genomic analyses in PDX model reveal a cyclin-dependent kinase inhibitor Palbociclib as a novel candidate drug for nasopharyngeal carcinoma

doi: 10.1186/s13046-018-0873-5

Figure Lengend Snippet: Genetic alterations in NPC-PDX tumors. ( a ) Copy number variations (CNV) of NPC-PDX tumors versus corresponding patient’s peripheral blood mononuclear cells (PBMC). Genome-wide CNV alterations in four paired PDX tumor samples (ST, LN, LG and LV). CCND1 CNV gain (red arrow) and CDKN2A CNV loss (green arrow) are indicated. ( b ) HE and EB virus-encoded small RNA (EBER) staining of parental NPC tumor with bone metastasis and its derived NPC-PDX. ( c ) CNV profile comparisons of NPC FFPE-Bone and PDX-Bone based on WES and ( d ) ultra-deep sequencing of cancer panel-409 are shown (genes associated with or without copy number alteration are indicated in different colors or in grey, respectively). Observed copy number for each evaluated position is shown on the y-axis as a log 2 scale. Correlation plots with Pearson’s correlation coefficient, r, indicating similarities between two CNV profiles

Article Snippet: Antibodies used in this study: RB1 (CusaBio PA003948), RB-P (Cell Signaling 9307), E2F1 (Santa Cruz SC-193), CDK2 (CusaBio PA001533), CDK4 (Santa Cruz SC-23896), CDK6 (Santa Cruz SC-53638), CCND1 (Santa Cruz SC-8396), CCNE2 (Proteintech 11,935–1-AP), CDKN2A (Prosci 4211), CDKN1A (Santa Cruz SC-6246), PCNA (Proteintech 10,205–2-AP) and GAPDH (Santa Cruz FL-335).

Techniques: Genome Wide, Virus, Staining, Derivative Assay, Sequencing

CCND1 mRNA expression and IHC staining in NPC patients and PDX tumors. ( a ) The expression fold change of candidate genes ( CCND1, CDKN2A and CDKN2B ) are indicated based on the cDNA microarray data of five PDX tissues, and C666–1 (EBV-positive NPC cells) and NP69 (immortalized normal nasopharyngeal cells, as control) cell lines. ( b ) Agarose gel electrophoresis of RT-PCR products of CCND1 in PBMC, two NPC cell lines and five PDXs (GAPDH serves as an internal control). Cyclin D1 IHC staining in ( c ) NPC no.13 patient, with NPC primary site, NPC metastatic to bone, and PDX-Bone tumor and ( d ) NPC no.2 patient, with NPC metastatic to lymph node, and PDX-LN tumor

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Integrated genomic analyses in PDX model reveal a cyclin-dependent kinase inhibitor Palbociclib as a novel candidate drug for nasopharyngeal carcinoma

doi: 10.1186/s13046-018-0873-5

Figure Lengend Snippet: CCND1 mRNA expression and IHC staining in NPC patients and PDX tumors. ( a ) The expression fold change of candidate genes ( CCND1, CDKN2A and CDKN2B ) are indicated based on the cDNA microarray data of five PDX tissues, and C666–1 (EBV-positive NPC cells) and NP69 (immortalized normal nasopharyngeal cells, as control) cell lines. ( b ) Agarose gel electrophoresis of RT-PCR products of CCND1 in PBMC, two NPC cell lines and five PDXs (GAPDH serves as an internal control). Cyclin D1 IHC staining in ( c ) NPC no.13 patient, with NPC primary site, NPC metastatic to bone, and PDX-Bone tumor and ( d ) NPC no.2 patient, with NPC metastatic to lymph node, and PDX-LN tumor

Article Snippet: Antibodies used in this study: RB1 (CusaBio PA003948), RB-P (Cell Signaling 9307), E2F1 (Santa Cruz SC-193), CDK2 (CusaBio PA001533), CDK4 (Santa Cruz SC-23896), CDK6 (Santa Cruz SC-53638), CCND1 (Santa Cruz SC-8396), CCNE2 (Proteintech 11,935–1-AP), CDKN2A (Prosci 4211), CDKN1A (Santa Cruz SC-6246), PCNA (Proteintech 10,205–2-AP) and GAPDH (Santa Cruz FL-335).

Techniques: Expressing, Immunohistochemistry, Microarray, Control, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction

Correlation of CNV of CCND1, CDKN2A and RAD52 with high EBV copy number in 22 NPC plasma. ( a ) CNV of CCND1 , CDKN2A and RAD52 in 22 NPC plasma with high EBV DNA load (> 5000 copies/ml) based on Q-PCR results. ( b ) Correlation plot between CNV of CCND1 , CDKN2A and RAD52 and log EBV DNA load in 22 NPC plasma samples ( c ) Correlation between CNV of the CCND1 / CDKN2A ratio and log EBV load in 22 NPC plasma. Pearson’s correlation coefficient, r, and equations of regression are indicated

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Integrated genomic analyses in PDX model reveal a cyclin-dependent kinase inhibitor Palbociclib as a novel candidate drug for nasopharyngeal carcinoma

doi: 10.1186/s13046-018-0873-5

Figure Lengend Snippet: Correlation of CNV of CCND1, CDKN2A and RAD52 with high EBV copy number in 22 NPC plasma. ( a ) CNV of CCND1 , CDKN2A and RAD52 in 22 NPC plasma with high EBV DNA load (> 5000 copies/ml) based on Q-PCR results. ( b ) Correlation plot between CNV of CCND1 , CDKN2A and RAD52 and log EBV DNA load in 22 NPC plasma samples ( c ) Correlation between CNV of the CCND1 / CDKN2A ratio and log EBV load in 22 NPC plasma. Pearson’s correlation coefficient, r, and equations of regression are indicated

Article Snippet: Antibodies used in this study: RB1 (CusaBio PA003948), RB-P (Cell Signaling 9307), E2F1 (Santa Cruz SC-193), CDK2 (CusaBio PA001533), CDK4 (Santa Cruz SC-23896), CDK6 (Santa Cruz SC-53638), CCND1 (Santa Cruz SC-8396), CCNE2 (Proteintech 11,935–1-AP), CDKN2A (Prosci 4211), CDKN1A (Santa Cruz SC-6246), PCNA (Proteintech 10,205–2-AP) and GAPDH (Santa Cruz FL-335).

Techniques: Clinical Proteomics

Comparison of non-structural protein 1 sequences among closely related flaviviruses. ( A ): Ribbon model highlighting regions of the NS1 protein containing segments exposed at the outer surface to the host environment. ( B ): Sequence comparison showing regions with high sequence disparity. Amino acids depicted in red differ from the corresponding ZIKV NS1 amino acids. A represents positions with two sequences with amino acids identical to ZIKV NS1. The boxes highlight highly conserved sequences, amino acids 117–119 and 227–229, that were mutated to alanine in immunodominant regions 2 and 3.

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: Comparison of non-structural protein 1 sequences among closely related flaviviruses. ( A ): Ribbon model highlighting regions of the NS1 protein containing segments exposed at the outer surface to the host environment. ( B ): Sequence comparison showing regions with high sequence disparity. Amino acids depicted in red differ from the corresponding ZIKV NS1 amino acids. A represents positions with two sequences with amino acids identical to ZIKV NS1. The boxes highlight highly conserved sequences, amino acids 117–119 and 227–229, that were mutated to alanine in immunodominant regions 2 and 3.

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Sequencing

Top ten pathways identified by pathway enrichment analysis for miR-30a and -191 .

Journal: PLoS ONE

Article Title: Overexpression of MicroRNA miR-30a or miR-191 in A549 Lung Cancer or BEAS-2B Normal Lung Cell Lines Does Not Alter Phenotype

doi: 10.1371/journal.pone.0009219

Figure Lengend Snippet: Top ten pathways identified by pathway enrichment analysis for miR-30a and -191 .

Article Snippet: BioCarta , Cyclins and Cell Cycle Regulation , 0.0013.

Techniques: Gene Expression, Control